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Potential Pitfalls and Solutions for Use of Fluorescent Fusion Proteins to Study the Lysosome
Use of fusion protein tags to investigate lysosomal proteins can be complicated by the acidic, protease-rich environment of the lysosome. Potential artifacts include degradation or release of the tag and acid quenching of fluorescence. Tagging can also affect protein folding, glycosylation and/or tr...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931630/ https://www.ncbi.nlm.nih.gov/pubmed/24586430 http://dx.doi.org/10.1371/journal.pone.0088893 |