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The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

INTRODUCTION: Determining bacterial community structure in fecal samples through DNA sequencing is an important facet of intestinal health research. The impact of different commercially available DNA extraction kits upon bacterial community structures has received relatively little attention. The ai...

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Detalles Bibliográficos
Autores principales: Kennedy, Nicholas A., Walker, Alan W., Berry, Susan H., Duncan, Sylvia H., Farquarson, Freda M., Louis, Petra, Thomson, John M., Satsangi, Jack, Flint, Harry J., Parkhill, Julian, Lees, Charlie W., Hold, Georgina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933346/
https://www.ncbi.nlm.nih.gov/pubmed/24586470
http://dx.doi.org/10.1371/journal.pone.0088982