Cargando…

The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing

INTRODUCTION: Determining bacterial community structure in fecal samples through DNA sequencing is an important facet of intestinal health research. The impact of different commercially available DNA extraction kits upon bacterial community structures has received relatively little attention. The ai...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kennedy, Nicholas A., Walker, Alan W., Berry, Susan H., Duncan, Sylvia H., Farquarson, Freda M., Louis, Petra, Thomson, John M., Satsangi, Jack, Flint, Harry J., Parkhill, Julian, Lees, Charlie W., Hold, Georgina L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933346/ https://www.ncbi.nlm.nih.gov/pubmed/24586470 http://dx.doi.org/10.1371/journal.pone.0088982

Ejemplares similares

The Impact of NOD2 Variants on Fecal Microbiota in Crohn’s Disease and Controls Without Gastrointestinal Disease
por: Kennedy, Nicholas A, et al.
Publicado: (2018)

16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice
por: Walker, Alan W., et al.
Publicado: (2015)

Wheat bran promotes enrichment within the human colonic microbiota of butyrate‐producing bacteria that release ferulic acid
por: Duncan, Sylvia H., et al.
Publicado: (2016)

Pivotal Roles for pH, Lactate, and Lactate-Utilizing Bacteria in the Stability of a Human Colonic Microbial Ecosystem
por: Wang, Shui Ping, et al.
Publicado: (2020)

Modulation of the human gut microbiota by dietary fibres occurs at the species level
por: Chung, Wing Sun Faith, et al.
Publicado: (2016)

Relative abundance of the Prevotella genus within the human gut microbiota of elderly volunteers determines the inter-individual responses to dietary supplementation with wheat bran arabinoxylan-oligosaccharides
por: Chung, Wing Sun Faith, et al.
Publicado: (2020)

Detection of Campylobacter concisus and Other Campylobacter Species in Colonic Biopsies from Adults with Ulcerative Colitis
por: Mukhopadhya, Indrani, et al.
Publicado: (2011)

Gut Mucosal Microbiome Signatures of Colorectal Cancer Differ According to BMI Status
por: Shaw, Sophie, et al.
Publicado: (2022)

Extending colonic mucosal microbiome analysis—assessment of colonic lavage as a proxy for endoscopic colonic biopsies
por: Watt, Euan, et al.
Publicado: (2016)

The Impact of NOD2 Genetic Variants on the Gut Mycobiota in Crohn’s Disease Patients in Remission and in Individuals Without Gastrointestinal Inflammation
por: Nelson, Andrew, et al.
Publicado: (2020)

A tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples
por: Dollive, Serena, et al.
Publicado: (2012)

THE EFFECT OF LABORATORY AGE UPON THE PHOTOTROPIC REACTIONS OF LIMULUS
por: Cole, William H.
Publicado: (1924)

Inter-Laboratory Assessment of a Prototype Multiplex Kit for Determination of Recent HIV-1 Infection
por: Curtis, Kelly A., et al.
Publicado: (2013)

Vitamin Biosynthesis by Human Gut Butyrate-Producing Bacteria and Cross-Feeding in Synthetic Microbial Communities
por: Soto-Martin, Eva C., et al.
Publicado: (2020)

Adjusting Coronavirus Prevalence Estimates for Laboratory Test Kit Error
por: Sempos, Christopher T, et al.
Publicado: (2020)

Thoracentesis Remarks upon Twenty Cases
por: Flint, Austin
Publicado: (1873)

The role of infection in the aetiology of inflammatory bowel disease
por: Hansen, Richard, et al.
Publicado: (2010)

Microbial degradation of complex carbohydrates in the gut
por: Flint, Harry J., et al.
Publicado: (2012)

Some are more equal than others: The role of “keystone” species in the degradation of recalcitrant substrates
por: Ze, Xiaolei, et al.
Publicado: (2013)

Clinical Utility And Diagnostic Accuracy of Faecal Calprotectin For IBD At First Presentation To Gastroenterology Services In Adults Aged 16–50 Years
por: Kennedy, Nicholas A., et al.
Publicado: (2015)

Laboratory evaluation of RealStar Yellow Fever Virus RT-PCR kit 1.0 for potential use in the global yellow fever laboratory network
por: Basile, Alison J., et al.
Publicado: (2022)

Emotional Arousal, Blood Glucose Levels, and Memory Modulation: Three Laboratory Exercises in Cognitive Neuroscience
por: Flint, Robert W.
Publicado: (2004)

The Pandora software development kit, CHEP 2012
por: Marshall, J S, et al.
Publicado: (2012)

Adobe ColdFusion 9, web application construction kit.
por: Forta, Ben, et al.
Publicado: (2011)

Adobe ColdFusion 9.: web application construction kit
por: Forta, Ben, et al.
Publicado: (2010)

Adobe ColdFusion 8.: web application construction kit
por: Forta, Ben, et al.
Publicado: (2007)

Elucidating biofilm diversity on water lily leaves through 16S rRNA amplicon analysis: Comparison of four DNA extraction kits
por: Janssen, Kathrin, et al.
Publicado: (2021)

Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification
por: Wagner, Josef, et al.
Publicado: (2016)

LMTK3 is essential for oncogenic KIT expression in KIT-mutant GIST and melanoma
por: Klug, Lillian R., et al.
Publicado: (2018)

Two-stage Genome-wide Methylation Profiling in Childhood-onset Crohn's Disease Implicates Epigenetic Alterations at the VMP1/MIR21 and HLA Loci
por: Adams, Alex T., et al.
Publicado: (2014)

Extracts from a Paper upon the Pathological Relations of the Gastric and Intestinal Tubes
por: Flint, Austin
Publicado: (1871)

Enrichment Preferences of FIV-Infected and Uninfected Laboratory-Housed Cats
por: Kennedy, Claudia J., et al.
Publicado: (2018)

Innate Immune Sensors and Gastrointestinal Bacterial Infections
por: Hold, Georgina L., et al.
Publicado: (2011)

Distribution, organization and expression of genes concerned with anaerobic lactate utilization in human intestinal bacteria
por: Sheridan, Paul O., et al.
Publicado: (2022)

Enterohepatic Helicobacter in Ulcerative Colitis: Potential Pathogenic Entities?
por: Thomson, John M., et al.
Publicado: (2011)

What’s in a commercial meal kit? Structured review of Australian meal kits
por: McKay, Fiona H
Publicado: (2023)

Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress
por: Liu, Xiaofeng, et al.
Publicado: (2018)

The Wasserman Test in the Municipal Laboratory and the Interpretation to Be Placed upon Results
por: Bissell, William G.
Publicado: (1914)

An Intergenic Non-Coding rRNA Correlated with Expression of the rRNA and Frequency of an rRNA Single Nucleotide Polymorphism in Lung Cancer Cells
por: Shiao, Yih-Horng, et al.
Publicado: (2009)

Bacterial Population Dynamics in a Laboratory Activated Sludge Reactor Monitored by Pyrosequencing of 16S rRNA
por: Satoh, Hiroyasu, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_v3t0nnnbdrhff9hjr9jlmm419o