Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation

Here we describe a fluctuation-based method to quantify how protein oligomerisation modulates signalling activity of a multifunctional protein. By recording fluorescence lifetime imaging microscopy (FLIM) data of a FRET biosensor in a format that enables concomitant phasor and cross Number and Brigh...

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Detalles Bibliográficos
Autores principales: Hinde, Elizabeth, Yokomori, Kyoko, Gaus, Katharina, Hahn, Klaus M., Gratton, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936235/
https://www.ncbi.nlm.nih.gov/pubmed/24573109
http://dx.doi.org/10.1038/srep04219

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936235/
https://www.ncbi.nlm.nih.gov/pubmed/24573109
http://dx.doi.org/10.1038/srep04219

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