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Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation

Here we describe a fluctuation-based method to quantify how protein oligomerisation modulates signalling activity of a multifunctional protein. By recording fluorescence lifetime imaging microscopy (FLIM) data of a FRET biosensor in a format that enables concomitant phasor and cross Number and Brigh...

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Detalles Bibliográficos
Autores principales:	Hinde, Elizabeth, Yokomori, Kyoko, Gaus, Katharina, Hahn, Klaus M., Gratton, Enrico
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936235/ https://www.ncbi.nlm.nih.gov/pubmed/24573109 http://dx.doi.org/10.1038/srep04219

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