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High-throughput, cost-effective verification of structural DNA assembly

DNA ‘assembly’ from ‘building blocks’ remains a cornerstone in synthetic biology, whether it be for gene synthesis (∼1 kb), pathway engineering (∼10 kb) or synthetic genomes (>100 kb). Despite numerous advances in the techniques used for DNA assembly, verification of the assembly is still a neces...

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Detalles Bibliográficos
Autores principales: Dharmadi, Yandi, Patel, Kedar, Shapland, Elaine, Hollis, Daniel, Slaby, Todd, Klinkner, Nicole, Dean, Jed, Chandran, Sunil S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936733/
https://www.ncbi.nlm.nih.gov/pubmed/24203706
http://dx.doi.org/10.1093/nar/gkt1088