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DNA polymerase preference determines PCR priming efficiency
BACKGROUND: Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of...
Autores principales: | Pan, Wenjing, Byrne-Steele, Miranda, Wang, Chunlin, Lu, Stanley, Clemmons, Scott, Zahorchak, Robert J, Han, Jian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937175/ https://www.ncbi.nlm.nih.gov/pubmed/24479830 http://dx.doi.org/10.1186/1472-6750-14-10 |
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