Cargando…

A Force-Based, Parallel Assay for the Quantification of Protein-DNA Interactions

Analysis of transcription factor binding to DNA sequences is of utmost importance to understand the intricate regulatory mechanisms that underlie gene expression. Several techniques exist that quantify DNA-protein affinity, but they are either very time-consuming or suffer from possible misinterpret...

Descripción completa

Detalles Bibliográficos
Autores principales:	Limmer, Katja, Pippig, Diana A., Aschenbrenner, Daniela, Gaub, Hermann E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3937344/ https://www.ncbi.nlm.nih.gov/pubmed/24586920 http://dx.doi.org/10.1371/journal.pone.0089626

Ejemplares similares

Parallel Force Assay for Protein-Protein Interactions
por: Aschenbrenner, Daniela, et al.
Publicado: (2014)

Sequence-specific inhibition of Dicer measured with a force-based microarray for RNA ligands
por: Limmer, Katja, et al.
Publicado: (2013)

Correction: Parallel Force Assay for Protein-Protein Interactions
Publicado: (2015)

Increasing evidence of mechanical force as a functional regulator in smooth muscle myosin light chain kinase
por: Baumann, Fabian, et al.
Publicado: (2017)

Different Vinculin Binding Sites Use the Same Mechanism to Regulate Directional Force Transduction
por: Kluger, Carleen, et al.
Publicado: (2020)

Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry
por: Sedlak, Steffen M., et al.
Publicado: (2017)

From Genes to Protein Mechanics on a Chip
por: Otten, Marcus, et al.
Publicado: (2014)

Ligand Binding Stabilizes Cellulosomal Cohesins as Revealed by AFM-based Single-Molecule Force Spectroscopy
por: Verdorfer, Tobias, et al.
Publicado: (2018)

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
por: Becke, Tanja D., et al.
Publicado: (2018)

Direct and site-specific quantification of RNA 2′-O-methylation by PCR with an engineered DNA polymerase
por: Aschenbrenner, Joos, et al.
Publicado: (2016)

Post-Translational Sortase-Mediated Attachment of High-Strength Force Spectroscopy Handles
por: Durner, Ellis, et al.
Publicado: (2017)

Ultrasensitive isolation, identification and quantification of DNA–protein adducts by ELISA-based RADAR assay
por: Kiianitsa, Kostantin, et al.
Publicado: (2014)

Mitochondrial Replication Assay (MIRA) for Efficient in situ Quantification of Nascent mtDNA and Protein Interactions with Nascent mtDNA (mitoSIRF)
por: Lozen, Macy, et al.
Publicado: (2023)

Proximity assays for sensitive quantification of proteins
por: Greenwood, Christina, et al.
Publicado: (2015)

An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions
por: de Jonge, Wim J., et al.
Publicado: (2020)

Development of Wrist Interface Based on Fully Actuated Coaxial Spherical Parallel Mechanism for Force Interaction
por: Lee, Jaeyong, et al.
Publicado: (2021)

PAIA assays: A new bead-based assay system for high throughput protein quantification
por: Wosnitza, Christine I, et al.
Publicado: (2015)

Cytosine methylation alters DNA mechanical properties
por: Severin, Philip M. D., et al.
Publicado: (2011)

Evaluation of a kDNA-Based qPCR Assay for the Detection and Quantification of Old World Leishmania Species
por: Ceccarelli, Marcello, et al.
Publicado: (2020)

Heterochromatin protein 1α interacts with parallel RNA and DNA G-quadruplexes
por: Roach, Ruby J, et al.
Publicado: (2020)

Mechanisms of Nanonewton Mechanostability in a Protein Complex Revealed by Molecular Dynamics Simulations and Single-Molecule Force Spectroscopy
por: Bernardi, Rafael C., et al.
Publicado: (2019)

A tethered ligand assay to probe SARS-CoV-2:ACE2 interactions
por: Bauer, Magnus S., et al.
Publicado: (2022)

Relative Quantification of Protein-Protein Interactions Using a Dual Luciferase Reporter Pull-Down Assay System
por: Jia, Shuaizheng, et al.
Publicado: (2011)

Quantification Assays for Total and Polyglutamine-Expanded Huntingtin Proteins
por: Macdonald, Douglas, et al.
Publicado: (2014)

Force-activated DNA substrates for probing individual proteins interacting with single-stranded DNA
por: Okoniewski, Stephen R., et al.
Publicado: (2017)

PEGylated surfaces for the study of DNA–protein interactions by atomic force microscopy
por: Akpinar, Bernice, et al.
Publicado: (2019)

Dual FRET assay for detecting receptor protein interaction with DNA
por: Krusiński, Tomasz, et al.
Publicado: (2010)

Bioorthogonal protein-DNA conjugation methods for force spectroscopy
por: Synakewicz, Marie, et al.
Publicado: (2019)

Moradas/50
por: Pippig, Alfredo
Publicado: (1975)

Calcium stabilizes the strongest protein fold
por: Milles, Lukas F., et al.
Publicado: (2018)

Parallel Microdispensing Method of High-Viscous Liquid Based on Electrostatic Force
por: Xu, Zheng, et al.
Publicado: (2022)

Precise protein quantification based on peptide quantification using iTRAQ™
por: Boehm, Andreas M, et al.
Publicado: (2007)

Bead based facile assay for sensitive quantification of native state green fluorescent protein
por: Kim, Jung Min, et al.
Publicado: (2020)

A tethered ligand assay to probe SARS-CoV-2:ACE2 interactions
por: Bauer, Magnus S., et al.
Publicado: (2022)

CMV DNA quantification in plasma using an automated real-time PCR assay
por: Yerly, S., et al.
Publicado: (2006)

Digital assay for rapid electronic quantification of clinical pathogens using DNA nanoballs
por: Tayyab, Muhammad, et al.
Publicado: (2023)

In Situ DNA/Protein Interaction Assay to Visualize Transcriptional Factor Activation
por: Corsini, Michela, et al.
Publicado: (2020)

A CAPS-based binding assay provides semi-quantitative validation of protein-DNA interactions
por: Xie, Yongyao, et al.
Publicado: (2016)

Interactive effects of personal resources and job characteristics on mental health: a population-based panel study
por: Limmer, Anja, et al.
Publicado: (2020)

Correction to: Interactive effects of personal resources and job characteristics on mental health: a population-based panel study
por: Limmer, Anja, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_jq1sl5vlu0g7u9jj09iqnjahso