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An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

BACKGROUND: To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing platforms. Sequencing low sequence diver...

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Detalles Bibliográficos
Autores principales: Fadrosh, Douglas W, Ma, Bing, Gajer, Pawel, Sengamalay, Naomi, Ott, Sandra, Brotman, Rebecca M, Ravel, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940169/
https://www.ncbi.nlm.nih.gov/pubmed/24558975
http://dx.doi.org/10.1186/2049-2618-2-6