Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase

Human granulocyte colony-stimulating factor (hGCSF), a neutrophil-promoting cytokine, is an effective therapeutic agent for neutropenia patients who have undergone several cancer treatments. Efficient production of hGCSF using E. coli is challenging because the hormone tends to aggregate and forms i...

Descripción completa

Detalles Bibliográficos
Autores principales: Do, Bich Hang, Ryu, Han-Bong, Hoang, Phuong, Koo, Bon-Kyung, Choe, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940694/
https://www.ncbi.nlm.nih.gov/pubmed/24594699
http://dx.doi.org/10.1371/journal.pone.0089906

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940694/
https://www.ncbi.nlm.nih.gov/pubmed/24594699
http://dx.doi.org/10.1371/journal.pone.0089906

Ejemplares similares