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A successful strategy for the recovering of active P21, an insoluble recombinant protein of Trypanosoma cruzi
Structural studies of proteins normally require large quantities of pure material that can only be obtained through heterologous expression systems and recombinant technique. In these procedures, large amounts of expressed protein are often found in the insoluble fraction, making protein purificatio...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941101/ https://www.ncbi.nlm.nih.gov/pubmed/24590372 http://dx.doi.org/10.1038/srep04259 |