Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation

Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterize human gut DC populations, and define their relationship to previously studied human and mouse DCs. CD103(+)Sirpα(−) DCs were related to human blood CD141(+) and to mouse intestinal CD103(+)CD11b(−...

Descripción completa

Detalles Bibliográficos
Autores principales: Watchmaker, Payal B., Lahl, Katharina, Lee, Mike, Baumjohann, Dirk, Morton, John, Kim, Sun Jung, Zeng, Ruizhu, Dent, Alexander, Ansel, K. Mark, Diamond, Betty, Hadeiba, Husein, Butcher, Eugene C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942165/
https://www.ncbi.nlm.nih.gov/pubmed/24292363
http://dx.doi.org/10.1038/ni.2768
_version_ 1782306040785666048
author Watchmaker, Payal B.
Lahl, Katharina
Lee, Mike
Baumjohann, Dirk
Morton, John
Kim, Sun Jung
Zeng, Ruizhu
Dent, Alexander
Ansel, K. Mark
Diamond, Betty
Hadeiba, Husein
Butcher, Eugene C.
author_facet Watchmaker, Payal B.
Lahl, Katharina
Lee, Mike
Baumjohann, Dirk
Morton, John
Kim, Sun Jung
Zeng, Ruizhu
Dent, Alexander
Ansel, K. Mark
Diamond, Betty
Hadeiba, Husein
Butcher, Eugene C.
author_sort Watchmaker, Payal B.
collection PubMed
description Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterize human gut DC populations, and define their relationship to previously studied human and mouse DCs. CD103(+)Sirpα(−) DCs were related to human blood CD141(+) and to mouse intestinal CD103(+)CD11b(−) DCs and expressed markers of cross-presenting DCs. CD103(+)Sirpα(+) DCs aligned with human blood CD1c(+) DCs and mouse intestinal CD103(+)CD11b(+) DCs and supported regulatory T cell induction. Both CD103(+) DC subsets induced T(H)17 cells, while CD103(−)Sirpα(+) DCs induced T(H)1 cells. Comparative transcriptomics revealed conserved transcriptional programs among CD103(+) DC subsets and uncovered a selective role for Bcl-6 and Blimp-1 in CD103(+)Sirpα(−) and intestinal CD103(+)CD11b(+) DC specification, respectively. These results highlight evolutionarily conserved and divergent programming of intestinal DCs.
format Online
Article
Text
id pubmed-3942165
institution National Center for Biotechnology Information
language English
publishDate 2013
record_format MEDLINE/PubMed
spelling pubmed-39421652014-07-01 Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation Watchmaker, Payal B. Lahl, Katharina Lee, Mike Baumjohann, Dirk Morton, John Kim, Sun Jung Zeng, Ruizhu Dent, Alexander Ansel, K. Mark Diamond, Betty Hadeiba, Husein Butcher, Eugene C. Nat Immunol Article Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterize human gut DC populations, and define their relationship to previously studied human and mouse DCs. CD103(+)Sirpα(−) DCs were related to human blood CD141(+) and to mouse intestinal CD103(+)CD11b(−) DCs and expressed markers of cross-presenting DCs. CD103(+)Sirpα(+) DCs aligned with human blood CD1c(+) DCs and mouse intestinal CD103(+)CD11b(+) DCs and supported regulatory T cell induction. Both CD103(+) DC subsets induced T(H)17 cells, while CD103(−)Sirpα(+) DCs induced T(H)1 cells. Comparative transcriptomics revealed conserved transcriptional programs among CD103(+) DC subsets and uncovered a selective role for Bcl-6 and Blimp-1 in CD103(+)Sirpα(−) and intestinal CD103(+)CD11b(+) DC specification, respectively. These results highlight evolutionarily conserved and divergent programming of intestinal DCs. 2013-12-01 2014-01 /pmc/articles/PMC3942165/ /pubmed/24292363 http://dx.doi.org/10.1038/ni.2768 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Watchmaker, Payal B.
Lahl, Katharina
Lee, Mike
Baumjohann, Dirk
Morton, John
Kim, Sun Jung
Zeng, Ruizhu
Dent, Alexander
Ansel, K. Mark
Diamond, Betty
Hadeiba, Husein
Butcher, Eugene C.
Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title_full Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title_fullStr Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title_full_unstemmed Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title_short Transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for Bcl-6 and Blimp-1 in terminal subset differentiation
title_sort transcriptional and functional profiling of human intestinal dendritic cells reveals conserved specialization and a role for bcl-6 and blimp-1 in terminal subset differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942165/
https://www.ncbi.nlm.nih.gov/pubmed/24292363
http://dx.doi.org/10.1038/ni.2768
work_keys_str_mv AT watchmakerpayalb transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT lahlkatharina transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT leemike transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT baumjohanndirk transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT mortonjohn transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT kimsunjung transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT zengruizhu transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT dentalexander transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT anselkmark transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT diamondbetty transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT hadeibahusein transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation
AT butchereugenec transcriptionalandfunctionalprofilingofhumanintestinaldendriticcellsrevealsconservedspecializationandaroleforbcl6andblimp1interminalsubsetdifferentiation

Ejemplares similares