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Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA
BACKGROUND: Due to their modular repeat structure, Pumilio/fem-3 mRNA binding factor (PUF) proteins are promising candidates for designer RNA-binding protein (RBP) engineering. To further facilitate the application of the PUF domain for the sequence-specific RBP engineering, a rapid cloning approach...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943411/ https://www.ncbi.nlm.nih.gov/pubmed/24581042 http://dx.doi.org/10.1186/1754-1611-8-7 |