C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells

We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format th...

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Detalles Bibliográficos
Autores principales: Zevnik, Branko, Uyttersprot, Nathalie C., Perez, Ana V., Bothe, Gerald W. M., Kern, Heidrun, Kauselmann, Gunther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944090/
https://www.ncbi.nlm.nih.gov/pubmed/24599260
http://dx.doi.org/10.1371/journal.pone.0090570
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author Zevnik, Branko
Uyttersprot, Nathalie C.
Perez, Ana V.
Bothe, Gerald W. M.
Kern, Heidrun
Kauselmann, Gunther
author_facet Zevnik, Branko
Uyttersprot, Nathalie C.
Perez, Ana V.
Bothe, Gerald W. M.
Kern, Heidrun
Kauselmann, Gunther
author_sort Zevnik, Branko
collection PubMed
description We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells.
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spelling pubmed-39440902014-03-10 C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells Zevnik, Branko Uyttersprot, Nathalie C. Perez, Ana V. Bothe, Gerald W. M. Kern, Heidrun Kauselmann, Gunther PLoS One Research Article We generated C57BL/6NTac mice carrying a tyrosinase loss-of function mutation and a reversion of the nonagouti locus to agouti. This strain has a high superovulation response, allows visual detection of chimeric coat color contribution of C57BL/6 ES-cells and provides a simplified breeding format that generates black G1 offspring of pure inbred C57BL/6 background in one step, providing the ideal host for genetically manipulated C57BL/6 ES cells. Public Library of Science 2014-03-05 /pmc/articles/PMC3944090/ /pubmed/24599260 http://dx.doi.org/10.1371/journal.pone.0090570 Text en © 2014 Zevnik et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zevnik, Branko
Uyttersprot, Nathalie C.
Perez, Ana V.
Bothe, Gerald W. M.
Kern, Heidrun
Kauselmann, Gunther
C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title_full C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title_fullStr C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title_full_unstemmed C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title_short C57BL/6N Albino/Agouti Mutant Mice as Embryo Donors for Efficient Germline Transmission of C57BL/6 ES Cells
title_sort c57bl/6n albino/agouti mutant mice as embryo donors for efficient germline transmission of c57bl/6 es cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944090/
https://www.ncbi.nlm.nih.gov/pubmed/24599260
http://dx.doi.org/10.1371/journal.pone.0090570
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