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A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal

Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of e...

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Detalles Bibliográficos
Autores principales: Zhang, Qiang, Jørgensen, Thomas J. D., Nielsen, Peter E., Møllegaard, Niels Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945016/
https://www.ncbi.nlm.nih.gov/pubmed/24599526
http://dx.doi.org/10.1371/journal.pone.0091138