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A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal
Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of e...
Autores principales: | Zhang, Qiang, Jørgensen, Thomas J. D., Nielsen, Peter E., Møllegaard, Niels Erik |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945016/ https://www.ncbi.nlm.nih.gov/pubmed/24599526 http://dx.doi.org/10.1371/journal.pone.0091138 |
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