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Transcription-generated torsional stress destabilizes nucleosomes

As RNA Polymerase II (Pol II) transcribes a gene, it encounters an array of well-ordered nucleosomes. How it traverses through this array in vivo remains unresolved. One model proposes that torsional stress generated during transcription destabilizes nucleosomes ahead of Pol II. Here, we describe a...

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Autores principales: Teves, Sheila S., Henikoff, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947361/
https://www.ncbi.nlm.nih.gov/pubmed/24317489
http://dx.doi.org/10.1038/nsmb.2723
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author Teves, Sheila S.
Henikoff, Steven
author_facet Teves, Sheila S.
Henikoff, Steven
author_sort Teves, Sheila S.
collection PubMed
description As RNA Polymerase II (Pol II) transcribes a gene, it encounters an array of well-ordered nucleosomes. How it traverses through this array in vivo remains unresolved. One model proposes that torsional stress generated during transcription destabilizes nucleosomes ahead of Pol II. Here, we describe a method for high resolution mapping of underwound DNA using next-generation sequencing, and show that torsion is correlated with gene expression in Drosophila melanogaster cells. Accumulation of torsional stress, through topoisomerase inhibition, results in increased. Pol II at transcription start sites. Whereas Topo I inhibition results in increased nascent RNA transcripts, Topo II inhibition shows little change. Despite the different effects on Pol II elongation, topoisomerase inhibition results in increased nucleosome turnover and salt solubility within gene bodies, suggesting that the elongation-independent effects of torsional stress on nucleosome dynamics contributes to the destabilization of nucleosomes.
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spelling pubmed-39473612014-07-01 Transcription-generated torsional stress destabilizes nucleosomes Teves, Sheila S. Henikoff, Steven Nat Struct Mol Biol Article As RNA Polymerase II (Pol II) transcribes a gene, it encounters an array of well-ordered nucleosomes. How it traverses through this array in vivo remains unresolved. One model proposes that torsional stress generated during transcription destabilizes nucleosomes ahead of Pol II. Here, we describe a method for high resolution mapping of underwound DNA using next-generation sequencing, and show that torsion is correlated with gene expression in Drosophila melanogaster cells. Accumulation of torsional stress, through topoisomerase inhibition, results in increased. Pol II at transcription start sites. Whereas Topo I inhibition results in increased nascent RNA transcripts, Topo II inhibition shows little change. Despite the different effects on Pol II elongation, topoisomerase inhibition results in increased nucleosome turnover and salt solubility within gene bodies, suggesting that the elongation-independent effects of torsional stress on nucleosome dynamics contributes to the destabilization of nucleosomes. 2013-12-08 2014-01 /pmc/articles/PMC3947361/ /pubmed/24317489 http://dx.doi.org/10.1038/nsmb.2723 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Teves, Sheila S.
Henikoff, Steven
Transcription-generated torsional stress destabilizes nucleosomes
title Transcription-generated torsional stress destabilizes nucleosomes
title_full Transcription-generated torsional stress destabilizes nucleosomes
title_fullStr Transcription-generated torsional stress destabilizes nucleosomes
title_full_unstemmed Transcription-generated torsional stress destabilizes nucleosomes
title_short Transcription-generated torsional stress destabilizes nucleosomes
title_sort transcription-generated torsional stress destabilizes nucleosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3947361/
https://www.ncbi.nlm.nih.gov/pubmed/24317489
http://dx.doi.org/10.1038/nsmb.2723
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