Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue

Enzyme stability is an important parameter in biocatalytic applications, and there is a strong need for efficient methods to generate robust enzymes. We investigated whether stabilizing disulfide bonds can be computationally designed based on a model structure. In our approach, unlike in previous di...

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Detalles Bibliográficos
Autores principales: van Beek, Hugo L., Wijma, Hein J., Fromont, Lucie, Janssen, Dick B., Fraaije, Marco W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953729/
https://www.ncbi.nlm.nih.gov/pubmed/24649397
http://dx.doi.org/10.1016/j.fob.2014.01.009

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3953729/
https://www.ncbi.nlm.nih.gov/pubmed/24649397
http://dx.doi.org/10.1016/j.fob.2014.01.009

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