A high-throughput approach for measuring temporal changes in the interactome

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches but these lack stoichiometric or temporal information. We combine quantitative proteomics and size exclusion chromatography to map 291 coeluting complexes. This method allows mapping...

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Detalles Bibliográficos
Autores principales: Kristensen, Anders R., Gsponer, Joerg, Foster, Leonard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954081/
https://www.ncbi.nlm.nih.gov/pubmed/22863883
http://dx.doi.org/10.1038/nmeth.2131
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author Kristensen, Anders R.
Gsponer, Joerg
Foster, Leonard J.
author_facet Kristensen, Anders R.
Gsponer, Joerg
Foster, Leonard J.
author_sort Kristensen, Anders R.
collection PubMed
description Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches but these lack stoichiometric or temporal information. We combine quantitative proteomics and size exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
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spelling pubmed-39540812014-03-14 A high-throughput approach for measuring temporal changes in the interactome Kristensen, Anders R. Gsponer, Joerg Foster, Leonard J. Nat Methods Article Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches but these lack stoichiometric or temporal information. We combine quantitative proteomics and size exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements. 2012-08-05 2012-09 /pmc/articles/PMC3954081/ /pubmed/22863883 http://dx.doi.org/10.1038/nmeth.2131 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kristensen, Anders R.
Gsponer, Joerg
Foster, Leonard J.
A high-throughput approach for measuring temporal changes in the interactome
title A high-throughput approach for measuring temporal changes in the interactome
title_full A high-throughput approach for measuring temporal changes in the interactome
title_fullStr A high-throughput approach for measuring temporal changes in the interactome
title_full_unstemmed A high-throughput approach for measuring temporal changes in the interactome
title_short A high-throughput approach for measuring temporal changes in the interactome
title_sort high-throughput approach for measuring temporal changes in the interactome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954081/
https://www.ncbi.nlm.nih.gov/pubmed/22863883
http://dx.doi.org/10.1038/nmeth.2131
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