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A high-throughput approach for measuring temporal changes in the interactome
Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches but these lack stoichiometric or temporal information. We combine quantitative proteomics and size exclusion chromatography to map 291 coeluting complexes. This method allows mapping...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954081/ https://www.ncbi.nlm.nih.gov/pubmed/22863883 http://dx.doi.org/10.1038/nmeth.2131 |