Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP

The transformation of the strain DH5α (TM)-T1(R) with plasmid vector pET11a containing the cloned gene of bacterial selenophosphate synthetase (SPS), selD, from the E. coli BL21-Gold (DE3) strain gives an overproducing strain of SPS with one synonymic substitution, E197D. The transformation efficien...

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Detalles Bibliográficos
Autores principales: Preobrazhenskaya, Y. V., Stenko, A. I., Shvarts, M. V., Lugovtsev, V. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956282/
https://www.ncbi.nlm.nih.gov/pubmed/24719756
http://dx.doi.org/10.1155/2013/983565

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956282/
https://www.ncbi.nlm.nih.gov/pubmed/24719756
http://dx.doi.org/10.1155/2013/983565

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