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True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells

Transcription activator-like effector nucleases (TALENs) are customizable fusion proteins able to cleave virtually any genomic DNA sequence of choice, and thereby to generate site-directed genetic modifications in a wide range of cells and organisms. In the present study, we expressed TALENs in poll...

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Detalles Bibliográficos
Autores principales: Gurushidze, Maia, Hensel, Goetz, Hiekel, Stefan, Schedel, Sindy, Valkov, Vladimir, Kumlehn, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958423/
https://www.ncbi.nlm.nih.gov/pubmed/24643227
http://dx.doi.org/10.1371/journal.pone.0092046