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shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo

Colon cancer is one of the most common tumors of the digestive tract. Resistance to ionizing radiation (IR) decreased therapeutic efficiency in these patients’ radiotherapy. XRCC2 is the key protein of DNA homologous recombination repair, and its high expression is associated with enhanced resistanc...

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Autores principales: Wang, Qin, Wang, Yan, Du, Liqing, Xu, Chang, Sun, Yuanming, Yang, Bing, Sun, Zhijuan, Fu, Yue, Cai, Lu, Fan, Saijun, Fan, Feiyue, Liu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958843/
https://www.ncbi.nlm.nih.gov/pubmed/24481064
http://dx.doi.org/10.3390/ijms15022157
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author Wang, Qin
Wang, Yan
Du, Liqing
Xu, Chang
Sun, Yuanming
Yang, Bing
Sun, Zhijuan
Fu, Yue
Cai, Lu
Fan, Saijun
Fan, Feiyue
Liu, Qiang
author_facet Wang, Qin
Wang, Yan
Du, Liqing
Xu, Chang
Sun, Yuanming
Yang, Bing
Sun, Zhijuan
Fu, Yue
Cai, Lu
Fan, Saijun
Fan, Feiyue
Liu, Qiang
author_sort Wang, Qin
collection PubMed
description Colon cancer is one of the most common tumors of the digestive tract. Resistance to ionizing radiation (IR) decreased therapeutic efficiency in these patients’ radiotherapy. XRCC2 is the key protein of DNA homologous recombination repair, and its high expression is associated with enhanced resistance to DNA damage induced by IR. Here, we investigated the effect of XRCC2 silencing on colon tumor cells’ growth and sensitivity to X-radiation in vitro and in vivo. Colon tumor cells (T84 cell line) were cultivated in vitro and tumors originated from the cell line were propagated as xenografts in nude mice. The suppression of XRCC2 expression was achieved by using vector-based short hairpin RNA (shRNA) in T84 cells. We found that the knockdown of XRCC2 expression effectively decreased T84 cellular proliferation and colony formation, and led to cell apoptosis and cell cycle arrested in G2/M phase induced by X-radiation in vitro. In addition, tumor xenograft studies suggested that XRCC2 silencing inhibited tumorigenicity after radiation treatment in vivo. Our data suggest that the suppression of XRCC2 expression rendered colon tumor cells more sensitive to radiation therapy in vitro and in vivo, implying XRCC2 as a promising therapeutic target for the treatment of radioresistant human colon cancer.
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spelling pubmed-39588432014-03-20 shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo Wang, Qin Wang, Yan Du, Liqing Xu, Chang Sun, Yuanming Yang, Bing Sun, Zhijuan Fu, Yue Cai, Lu Fan, Saijun Fan, Feiyue Liu, Qiang Int J Mol Sci Article Colon cancer is one of the most common tumors of the digestive tract. Resistance to ionizing radiation (IR) decreased therapeutic efficiency in these patients’ radiotherapy. XRCC2 is the key protein of DNA homologous recombination repair, and its high expression is associated with enhanced resistance to DNA damage induced by IR. Here, we investigated the effect of XRCC2 silencing on colon tumor cells’ growth and sensitivity to X-radiation in vitro and in vivo. Colon tumor cells (T84 cell line) were cultivated in vitro and tumors originated from the cell line were propagated as xenografts in nude mice. The suppression of XRCC2 expression was achieved by using vector-based short hairpin RNA (shRNA) in T84 cells. We found that the knockdown of XRCC2 expression effectively decreased T84 cellular proliferation and colony formation, and led to cell apoptosis and cell cycle arrested in G2/M phase induced by X-radiation in vitro. In addition, tumor xenograft studies suggested that XRCC2 silencing inhibited tumorigenicity after radiation treatment in vivo. Our data suggest that the suppression of XRCC2 expression rendered colon tumor cells more sensitive to radiation therapy in vitro and in vivo, implying XRCC2 as a promising therapeutic target for the treatment of radioresistant human colon cancer. Molecular Diversity Preservation International (MDPI) 2014-01-29 /pmc/articles/PMC3958843/ /pubmed/24481064 http://dx.doi.org/10.3390/ijms15022157 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wang, Qin
Wang, Yan
Du, Liqing
Xu, Chang
Sun, Yuanming
Yang, Bing
Sun, Zhijuan
Fu, Yue
Cai, Lu
Fan, Saijun
Fan, Feiyue
Liu, Qiang
shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title_full shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title_fullStr shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title_full_unstemmed shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title_short shRNA-Mediated XRCC2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells to X-ray Irradiation in Vitro and in Vivo
title_sort shrna-mediated xrcc2 gene knockdown efficiently sensitizes colon tumor cells to x-ray irradiation in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958843/
https://www.ncbi.nlm.nih.gov/pubmed/24481064
http://dx.doi.org/10.3390/ijms15022157
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