Combination of FACS and Homologous Recombination for the Generation of Stable and High-Expression Engineered Cell Lines

Traditionally, cell line generation requires several months and involves screening of over several hundred cell clones for high productivity before dozens are selected as candidate cell lines. Here, we have designed a new strategy for the generation of stable and high-expression cell lines by combin...

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Detalles Bibliográficos
Autores principales: Shi, Lei, Chen, Xuesi, Tang, Wenying, Li, Zhenyi, Liu, Jin, Gao, Feng, Sang, Jianli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960159/
https://www.ncbi.nlm.nih.gov/pubmed/24646904
http://dx.doi.org/10.1371/journal.pone.0091712

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960159/
https://www.ncbi.nlm.nih.gov/pubmed/24646904
http://dx.doi.org/10.1371/journal.pone.0091712

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