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Combination of FACS and Homologous Recombination for the Generation of Stable and High-Expression Engineered Cell Lines
Traditionally, cell line generation requires several months and involves screening of over several hundred cell clones for high productivity before dozens are selected as candidate cell lines. Here, we have designed a new strategy for the generation of stable and high-expression cell lines by combin...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960159/ https://www.ncbi.nlm.nih.gov/pubmed/24646904 http://dx.doi.org/10.1371/journal.pone.0091712 |