An Extended ΔCT-Method Facilitating Normalisation with Multiple Reference Genes Suited for Quantitative RT-PCR Analyses of Human Hepatocyte-Like Cells

Reference genes (RG) as sample internal controls for gene transcript level analyses by quantitative RT-PCR (RT-qPCR) must be stably expressed within the experimental range. A variety of in vitro cell culture settings with primary human hepatocytes, and Huh-7 and HepG2 cell lines, were used to determ...

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Detalles Bibliográficos
Autores principales: Riedel, Gesa, Rüdrich, Urda, Fekete-Drimusz, Nora, Manns, Michael P., Vondran, Florian W. R., Bock, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962476/
https://www.ncbi.nlm.nih.gov/pubmed/24658132
http://dx.doi.org/10.1371/journal.pone.0093031

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962476/
https://www.ncbi.nlm.nih.gov/pubmed/24658132
http://dx.doi.org/10.1371/journal.pone.0093031

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