Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity

The discovery of microRNAs (miRNAs), which are ∼21–23 nucleotides that can regulate targeted mRNA by transcript cleavage or protein translation suppression, has changed the landscape of biomedical field greatly. At present, Northern blot analysis based on radioisotopes is still the most popular meth...

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Detalles Bibliográficos
Autores principales: Wu, Wei, Gong, Pengtao, Li, Jianhua, Yang, Ju, Zhang, Guocai, Li, He, Yang, Zhengtao, Zhang, Xichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964919/
https://www.ncbi.nlm.nih.gov/pubmed/24572812
http://dx.doi.org/10.1261/rna.042150.113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964919/
https://www.ncbi.nlm.nih.gov/pubmed/24572812
http://dx.doi.org/10.1261/rna.042150.113

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