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Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
The discovery of microRNAs (miRNAs), which are ∼21–23 nucleotides that can regulate targeted mRNA by transcript cleavage or protein translation suppression, has changed the landscape of biomedical field greatly. At present, Northern blot analysis based on radioisotopes is still the most popular meth...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964919/ https://www.ncbi.nlm.nih.gov/pubmed/24572812 http://dx.doi.org/10.1261/rna.042150.113 |