Efficient Designer Nuclease-Based Homologous Recombination Enables Direct PCR Screening for Footprintless Targeted Human Pluripotent Stem Cells

Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer nucleases has been shown to be significantly more efficient than conventional gene targeting, but still typically depends on the introduction of additional genetic selection elements. In our study, we demons...

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Detalles Bibliográficos
Autores principales: Merkert, Sylvia, Wunderlich, Stephanie, Bednarski, Christien, Beier, Jennifer, Haase, Alexandra, Dreyer, Anne-Kathrin, Schwanke, Kristin, Meyer, Johann, Göhring, Gudrun, Cathomen, Toni, Martin, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966116/
https://www.ncbi.nlm.nih.gov/pubmed/24678453
http://dx.doi.org/10.1016/j.stemcr.2013.12.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966116/
https://www.ncbi.nlm.nih.gov/pubmed/24678453
http://dx.doi.org/10.1016/j.stemcr.2013.12.003

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