Cargando…

Efficient Designer Nuclease-Based Homologous Recombination Enables Direct PCR Screening for Footprintless Targeted Human Pluripotent Stem Cells

Genetic engineering of human induced pluripotent stem cells (hiPSCs) via customized designer nucleases has been shown to be significantly more efficient than conventional gene targeting, but still typically depends on the introduction of additional genetic selection elements. In our study, we demons...

Descripción completa

Detalles Bibliográficos
Autores principales:	Merkert, Sylvia, Wunderlich, Stephanie, Bednarski, Christien, Beier, Jennifer, Haase, Alexandra, Dreyer, Anne-Kathrin, Schwanke, Kristin, Meyer, Johann, Göhring, Gudrun, Cathomen, Toni, Martin, Ulrich
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2014
Materias:	Resource
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966116/ https://www.ncbi.nlm.nih.gov/pubmed/24678453 http://dx.doi.org/10.1016/j.stemcr.2013.12.003

Ejemplares similares

Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture
por: Olmer, Ruth, et al.
Publicado: (2018)

Homology Requirements for Efficient, Footprintless Gene Editing at the CFTR Locus in Human iPSCs with Helper-dependent Adenoviral Vectors
por: Palmer, Donna J, et al.
Publicado: (2016)

OutKnocker: a web tool for rapid and simple genotyping of designer nuclease edited cell lines
por: Schmid-Burgk, Jonathan L., et al.
Publicado: (2014)

Targeted Integration of a Super-Exon into the CFTR Locus Leads to Functional Correction of a Cystic Fibrosis Cell Line Model
por: Bednarski, Christien, et al.
Publicado: (2016)

Homologous recombination mediates stable Fah gene integration and phenotypic correction in tyrosinaemia mouse-model
por: Junge, Norman, et al.
Publicado: (2018)

High-Throughput Screening for Modulators of CFTR Activity Based on Genetically Engineered Cystic Fibrosis Disease-Specific iPSCs
por: Merkert, Sylvia, et al.
Publicado: (2019)

Defining the nature of human pluripotent stem cell-derived interneurons via single-cell analysis
por: Allison, Thomas, et al.
Publicado: (2021)

Molecular Criteria for Defining the Naive Human Pluripotent State
por: Theunissen, Thorold W., et al.
Publicado: (2016)

Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance
por: Zaidan, Nur Zafirah, et al.
Publicado: (2018)

Gastruloid Development Competence Discriminates Different States of Pluripotency
por: Cermola, Federica, et al.
Publicado: (2021)

Nutritional requirements of human induced pluripotent stem cells
por: Lyra-Leite, Davi M., et al.
Publicado: (2023)

Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells
por: Baker, Duncan, et al.
Publicado: (2016)

Site-Specific Genome Engineering in Human Pluripotent Stem Cells
por: Merkert, Sylvia, et al.
Publicado: (2016)

Detecting Sequence Homology at the Gene Cluster Level with MultiGeneBlast
por: Medema, Marnix H., et al.
Publicado: (2013)

Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells
por: Siller, Richard, et al.
Publicado: (2015)

KLF4 N-Terminal Variance Modulates Induced Reprogramming to Pluripotency
por: Kim, Shin-Il, et al.
Publicado: (2015)

The pluripotent regulatory circuitry connecting promoters to their long-range interacting elements
por: Schoenfelder, Stefan, et al.
Publicado: (2015)

DAZL Limits Pluripotency, Differentiation, and Apoptosis in Developing Primordial Germ Cells
por: Chen, Hsu-Hsin, et al.
Publicado: (2014)

GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives
por: Kao, Tim, et al.
Publicado: (2016)

Chemically Defined Media Can Maintain Pig Pluripotency Network In Vitro
por: Choi, Kwang-Hwan, et al.
Publicado: (2019)

Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
por: Kooreman, Nigel G., et al.
Publicado: (2017)

High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells
por: D'Antonio, Matteo, et al.
Publicado: (2017)

A Stem Cell Reporter for Investigating Pluripotency and Self-Renewal in the Rat
por: Meek, Stephen, et al.
Publicado: (2020)

Scalable generation of sensory neurons from human pluripotent stem cells
por: Deng, Tao, et al.
Publicado: (2023)

Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis
por: Boroviak, Thorsten, et al.
Publicado: (2015)

Single Cell RNA-Sequencing of Pluripotent States Unlocks Modular Transcriptional Variation
por: Kolodziejczyk, Aleksandra A., et al.
Publicado: (2015)

Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (Phospho)Proteomic Profiling
por: Singec, Ilyas, et al.
Publicado: (2016)

Generation of pancreatic progenitors from human pluripotent stem cells by small molecules
por: Jiang, Yuqian, et al.
Publicado: (2021)

Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells
por: Tristan, Carlos A., et al.
Publicado: (2021)

Human pluripotent stem cell-derived macrophages host Mycobacterium abscessus infection
por: Sun, Shicheng, et al.
Publicado: (2022)

Modeling sex differences in humans using isogenic induced pluripotent stem cells
por: Waldhorn, Ithai, et al.
Publicado: (2022)

Analysis of Induced Pluripotent Stem Cells from a BRCA1 Mutant Family
por: Soyombo, Abigail A., et al.
Publicado: (2013)

Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture
por: Kempf, Henning, et al.
Publicado: (2014)

Aberrant α-Adrenergic Hypertrophic Response in Cardiomyocytes from Human Induced Pluripotent Cells
por: Földes, Gabor, et al.
Publicado: (2014)

Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection
por: Steyer, Benjamin, et al.
Publicado: (2018)

Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells
por: Kauts, Mari-Liis, et al.
Publicado: (2018)

Nonadhesive Alginate Hydrogels Support Growth of Pluripotent Stem Cell-Derived Intestinal Organoids
por: Capeling, Meghan M., et al.
Publicado: (2019)

Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells
por: Theurillat, Ilan, et al.
Publicado: (2020)

A Comprehensive Resource for Induced Pluripotent Stem Cells from Patients with Primary Tauopathies
por: Karch, Celeste M., et al.
Publicado: (2019)

Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
por: Bredenkamp, Nicholas, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_dl8b0uc1clvojjlkdl015d6400