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Discovering Aptamers by Cell-SELEX against Human Soluble Growth Factors Ectopically Expressed on Yeast Cell Surface
SELEX, the process of selecting aptamers, is often hampered by the difficulty of preparing target molecules in their native forms and by a lack of a simple yet quantitative assay for monitoring enrichment and affinity of reactive aptamers. In this study, we sought to discover DNA aptamers against hu...
Autores principales: | Meng, Hsien-Wei, Pagano, John M., White, Brian S., Toyoda, Yoshiko, Min, Irene M., Craighead, Harold G., Shalloway, David, Lis, John T., Xiao, Kai, Jin, Moonsoo M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968096/ https://www.ncbi.nlm.nih.gov/pubmed/24675636 http://dx.doi.org/10.1371/journal.pone.0093052 |
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