Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection

Single-stranded DNA (ssDNA) is a prerequisite for electrochemical sensor-based detection of parasite DNA and other diagnostic applications. To achieve this detection, an asymmetric polymerase chain reaction method was optimised. This method facilitates amplification of ssDNA from the human lymphatic...

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Detalles Bibliográficos
Autores principales: Venkatesan, Vasuki, Hoti, Sugeerappa Laxmanappa, Kamaraj, Nagalakshmi, Ghosh, Somnath, Rajaram, Kaushik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2013
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970695/
https://www.ncbi.nlm.nih.gov/pubmed/24037206
http://dx.doi.org/10.1590/0074-0276108062013020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970695/
https://www.ncbi.nlm.nih.gov/pubmed/24037206
http://dx.doi.org/10.1590/0074-0276108062013020

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