Cargando…

Three-Step Method for Proliferation and Differentiation of Human Embryonic Stem Cell (hESC)-Derived Male Germ Cells

The low efficiency of differentiation into male germ cell (GC)-like cells and haploid germ cells from human embryonic stem cells (hESCs) reflects the culture method employed in the two-dimensional (2D)-microenvironment. In this study, we applied a three-step media and calcium alginate-based 3D-cultu...

Descripción completa

Detalles Bibliográficos
Autores principales: Lim, Jung Jin, Shim, Myung Sun, Lee, Jeoung Eun, Lee, Dong Ryul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972183/
https://www.ncbi.nlm.nih.gov/pubmed/24690677
http://dx.doi.org/10.1371/journal.pone.0090454