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Isolation and Validation of an Endogenous Fluorescent Nucleoid Reporter in Salmonella Typhimurium

In this study we adapted a Mud-based delivery system to construct a random yfp reporter gene (encoding the yellow fluorescent protein) insertion library in the genome of Salmonella Typhimurium LT2, and used fluorescence activated cell sorting and fluorescence microscopy to screen for translational f...

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Detalles Bibliográficos
Autores principales: Passaris, Ioannis, Ghosh, Anirban, Cenens, William, Michiels, Chris W., Lammertyn, Jeroen, Aertsen, Abram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973593/
https://www.ncbi.nlm.nih.gov/pubmed/24695782
http://dx.doi.org/10.1371/journal.pone.0093785