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Isolation and Validation of an Endogenous Fluorescent Nucleoid Reporter in Salmonella Typhimurium
In this study we adapted a Mud-based delivery system to construct a random yfp reporter gene (encoding the yellow fluorescent protein) insertion library in the genome of Salmonella Typhimurium LT2, and used fluorescence activated cell sorting and fluorescence microscopy to screen for translational f...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973593/ https://www.ncbi.nlm.nih.gov/pubmed/24695782 http://dx.doi.org/10.1371/journal.pone.0093785 |