H2A.B facilitates transcription elongation at methylated CpG loci

H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are activ...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Yibin, Chen, Qiang, McEachin, Richard C., Cavalcoli, James D., Yu, Xiaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975057/
https://www.ncbi.nlm.nih.gov/pubmed/24402521
http://dx.doi.org/10.1101/gr.156877.113
_version_ 1782310076715892736
author Chen, Yibin
Chen, Qiang
McEachin, Richard C.
Cavalcoli, James D.
Yu, Xiaochun
author_facet Chen, Yibin
Chen, Qiang
McEachin, Richard C.
Cavalcoli, James D.
Yu, Xiaochun
author_sort Chen, Yibin
collection PubMed
description H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are actively transcribed. One typical example is that H2A.B is enriched in a set of differentially methylated regions at imprinted loci and facilitates transcription elongation. These results suggest that H2A.B positively regulates transcription elongation by overcoming DNA methylation in the transcribed region. It provides a novel mechanism by which transcription is regulated at DNA hypermethylated regions.
format Online
Article
Text
id pubmed-3975057
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-39750572014-10-01 H2A.B facilitates transcription elongation at methylated CpG loci Chen, Yibin Chen, Qiang McEachin, Richard C. Cavalcoli, James D. Yu, Xiaochun Genome Res Research H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are actively transcribed. One typical example is that H2A.B is enriched in a set of differentially methylated regions at imprinted loci and facilitates transcription elongation. These results suggest that H2A.B positively regulates transcription elongation by overcoming DNA methylation in the transcribed region. It provides a novel mechanism by which transcription is regulated at DNA hypermethylated regions. Cold Spring Harbor Laboratory Press 2014-04 /pmc/articles/PMC3975057/ /pubmed/24402521 http://dx.doi.org/10.1101/gr.156877.113 Text en © 2014 Chen et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.
spellingShingle Research
Chen, Yibin
Chen, Qiang
McEachin, Richard C.
Cavalcoli, James D.
Yu, Xiaochun
H2A.B facilitates transcription elongation at methylated CpG loci
title H2A.B facilitates transcription elongation at methylated CpG loci
title_full H2A.B facilitates transcription elongation at methylated CpG loci
title_fullStr H2A.B facilitates transcription elongation at methylated CpG loci
title_full_unstemmed H2A.B facilitates transcription elongation at methylated CpG loci
title_short H2A.B facilitates transcription elongation at methylated CpG loci
title_sort h2a.b facilitates transcription elongation at methylated cpg loci
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975057/
https://www.ncbi.nlm.nih.gov/pubmed/24402521
http://dx.doi.org/10.1101/gr.156877.113
work_keys_str_mv AT chenyibin h2abfacilitatestranscriptionelongationatmethylatedcpgloci
AT chenqiang h2abfacilitatestranscriptionelongationatmethylatedcpgloci
AT mceachinrichardc h2abfacilitatestranscriptionelongationatmethylatedcpgloci
AT cavalcolijamesd h2abfacilitatestranscriptionelongationatmethylatedcpgloci
AT yuxiaochun h2abfacilitatestranscriptionelongationatmethylatedcpgloci

Ejemplares similares