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Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats
BACKGROUND: Altered hypothalamo-pituitary-adrenal (HPA) axis activity may be accompanied by a modulation of pain sensitivity. In a model of neuropathic pain (chronic constriction injury, CCI) we investigated the onset and maintenance of mechanical allodynia/hyperalgesia and the expression of biochem...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975939/ https://www.ncbi.nlm.nih.gov/pubmed/24575861 http://dx.doi.org/10.1186/1471-2202-15-35 |
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author | Le Coz, Glenn-Marie Fiatte, Cathy Anton, Fernand Hanesch, Ulrike |
author_facet | Le Coz, Glenn-Marie Fiatte, Cathy Anton, Fernand Hanesch, Ulrike |
author_sort | Le Coz, Glenn-Marie |
collection | PubMed |
description | BACKGROUND: Altered hypothalamo-pituitary-adrenal (HPA) axis activity may be accompanied by a modulation of pain sensitivity. In a model of neuropathic pain (chronic constriction injury, CCI) we investigated the onset and maintenance of mechanical allodynia/hyperalgesia and the expression of biochemical mediators potentially involved in spinal cell modulation in two rat strains displaying either hypo- (Lewis-LEW) or hyper- (Fischer 344-FIS) reactivity of the HPA axis. RESULTS: Mechanical pain thresholds and plasmatic corticosterone levels were assessed before and during periods of 4 or 21 days following CCI surgery. At the end of the respective protocols, the mRNA expression of glial cell markers (GFAP and Iba1) and glutamate transporters (EAAT3 and EAAT2) were examined. We observed a correlation between the HPA axis reactivity and the pain behavior but not as commonly described in the literature; LEW rats seemed to be less sensitive than FIS from 4 to 14 days after the CCI surgery when looking at the mechanical allodynia/hyperalgesia. However, the biochemical spinal markers expression we observed is conflicting. CONCLUSION: We did not find a specific causal relation between the pain behavior and the glial cell activation or the expression of the glutamate transporters, suggesting that the interaction between the HPA axis and the spinal activation pattern is more complex in a context of neuropathic pain. |
format | Online Article Text |
id | pubmed-3975939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39759392014-04-05 Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats Le Coz, Glenn-Marie Fiatte, Cathy Anton, Fernand Hanesch, Ulrike BMC Neurosci Research Article BACKGROUND: Altered hypothalamo-pituitary-adrenal (HPA) axis activity may be accompanied by a modulation of pain sensitivity. In a model of neuropathic pain (chronic constriction injury, CCI) we investigated the onset and maintenance of mechanical allodynia/hyperalgesia and the expression of biochemical mediators potentially involved in spinal cell modulation in two rat strains displaying either hypo- (Lewis-LEW) or hyper- (Fischer 344-FIS) reactivity of the HPA axis. RESULTS: Mechanical pain thresholds and plasmatic corticosterone levels were assessed before and during periods of 4 or 21 days following CCI surgery. At the end of the respective protocols, the mRNA expression of glial cell markers (GFAP and Iba1) and glutamate transporters (EAAT3 and EAAT2) were examined. We observed a correlation between the HPA axis reactivity and the pain behavior but not as commonly described in the literature; LEW rats seemed to be less sensitive than FIS from 4 to 14 days after the CCI surgery when looking at the mechanical allodynia/hyperalgesia. However, the biochemical spinal markers expression we observed is conflicting. CONCLUSION: We did not find a specific causal relation between the pain behavior and the glial cell activation or the expression of the glutamate transporters, suggesting that the interaction between the HPA axis and the spinal activation pattern is more complex in a context of neuropathic pain. BioMed Central 2014-02-28 /pmc/articles/PMC3975939/ /pubmed/24575861 http://dx.doi.org/10.1186/1471-2202-15-35 Text en Copyright © 2014 Le Coz et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Le Coz, Glenn-Marie Fiatte, Cathy Anton, Fernand Hanesch, Ulrike Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title | Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title_full | Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title_fullStr | Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title_full_unstemmed | Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title_short | Differential neuropathic pain sensitivity and expression of spinal mediators in Lewis and Fischer 344 rats |
title_sort | differential neuropathic pain sensitivity and expression of spinal mediators in lewis and fischer 344 rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975939/ https://www.ncbi.nlm.nih.gov/pubmed/24575861 http://dx.doi.org/10.1186/1471-2202-15-35 |
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