Challenges to Design and Develop of DNA Aptamers for Protein Targets. I. Optimization of Asymmetric PCR for Generation of a Single Stranded DNA Library

Aptamers, or single stranded oligonucleotides, are produced by systematic evolution of ligands by exponential enrichment, abbreviated as SELEX. In the amplification and regeneration step of SELEX technique, dsDNA is conversed to ssDNA. Asymmetric PCR is one of the methods used for the generation of...

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Detalles Bibliográficos
Autores principales: Tabarzad, Maryam, Kazemi, Bahram, Vahidi, Hossein, Aboofazeli, Reza, Shahhosseini, Soraya, Nafissi-Varcheh, Nastaran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977063/
https://www.ncbi.nlm.nih.gov/pubmed/24711839

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977063/
https://www.ncbi.nlm.nih.gov/pubmed/24711839

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