Negative selection and stringency modulation in phage-assisted constinuous evolution

Phage-assisted continuous evolution (PACE) uses a modified filamentous bacteriophage life cycle to dramatically accelerate laboratory evolution experiments. In this work we expand the scope and capabilities of the PACE method with two key advances that enable the evolution of biomolecules with radic...

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Detalles Bibliográficos
Autores principales: Carlson, Jacob C., Badran, Ahmed H., Guggiana-Nilo, Drago A., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977541/
https://www.ncbi.nlm.nih.gov/pubmed/24487694
http://dx.doi.org/10.1038/nchembio.1453

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977541/
https://www.ncbi.nlm.nih.gov/pubmed/24487694
http://dx.doi.org/10.1038/nchembio.1453

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