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A New Assay for Determining Ganglioside Sialyltransferase Activities Lactosylceramide-2,3-Sialyltransferase (SAT I) and Monosialylganglioside-2,3-Sialyltransferase (SAT IV)

A new assay for the determination of lactosylceramide-2,3-sialyltransferase (SAT I, EC 2.4.99.9) and monosialoganglioside sialyltransferase (SAT IV, EC 2.4.99.2) is described. The assay utilised the commercially available fluorophore labelled sphingolipids, boron dipyrromethene difluoride (BODIPY) l...

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Detalles Bibliográficos
Autores principales: Sun, Cynthia Q., Hubl, Ulrike, Hoefakker, Petra, Vasudevamurthy, Madhusudan K., Johnson, Keryn D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981761/
https://www.ncbi.nlm.nih.gov/pubmed/24718572
http://dx.doi.org/10.1371/journal.pone.0094206