Crystal structure of the trithorax group protein Ash2L reveals a Forkhead-like DNA binding domain

Human ASH2L is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that Ash2L binds DNA employing a Forkhead-like helix-wing-helix (HWH) domain. In vivo, Ash2L HWH domain is required for binding to the β-globin locus control regio...

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Detalles Bibliográficos
Autores principales: Sarvan, Sabina, Avdic, Vanja, Tremblay, Véronique, Chaturvedi, Chandra-Prakash, Zhang, Pamela, Lanouette, Sylvain, Blais, Alexandre, Brunzelle, Joseph S., Brand, Marjorie, Couture, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983046/
https://www.ncbi.nlm.nih.gov/pubmed/21642971
http://dx.doi.org/10.1038/nsmb.2093
Descripción
Sumario:Human ASH2L is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that Ash2L binds DNA employing a Forkhead-like helix-wing-helix (HWH) domain. In vivo, Ash2L HWH domain is required for binding to the β-globin locus control region (LCR), histone H3 Lys4 tri-methylation and maximal expression of the β-globin gene, validating the functional importance of Ash2L DNA binding activity.

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