A 5000-Fold Increase in the Specificity of a Bacterial Phosphotriesterase for Malathion through Combinatorial Active Site Mutagenesis

Phosphotriesterases (PTEs) have been isolated from a range of bacterial species, including Agrobcaterium radiobacter (PTE(Ar)), and are efficient enzymes with broad substrate ranges. The turnover rate of PTE(Ar) for the common organophosphorous insecticide malathion is lower than expected based on i...

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Detalles Bibliográficos
Autores principales: Naqvi, Tatheer, Warden, Andrew C., French, Nigel, Sugrue, Elena, Carr, Paul D., Jackson, Colin J., Scott, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983157/
https://www.ncbi.nlm.nih.gov/pubmed/24721933
http://dx.doi.org/10.1371/journal.pone.0094177

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983157/
https://www.ncbi.nlm.nih.gov/pubmed/24721933
http://dx.doi.org/10.1371/journal.pone.0094177

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