Elimination of Ligation Dependent Artifacts in T4 RNA Ligase to Achieve High Efficiency and Low Bias MicroRNA Capture

Adapter ligation is a critical first step in many microRNA analysis methods including microarray, qPCR, and sequencing. Previous studies have shown that ligation bias can have dramatic effects on both the fidelity of expression profiles and reproducibility across samples. We have developed a method...

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Detalles Bibliográficos
Autores principales: Song, Yunke, Liu, Kelvin J., Wang, Tza-Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983213/
https://www.ncbi.nlm.nih.gov/pubmed/24722341
http://dx.doi.org/10.1371/journal.pone.0094619

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983213/
https://www.ncbi.nlm.nih.gov/pubmed/24722341
http://dx.doi.org/10.1371/journal.pone.0094619

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