A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR

Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA puri...

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Detalles Bibliográficos
Autores principales: Shatzkes, Kenneth, Teferedegne, Belete, Murata, Haruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983595/
https://www.ncbi.nlm.nih.gov/pubmed/24722424
http://dx.doi.org/10.1038/srep04659

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983595/
https://www.ncbi.nlm.nih.gov/pubmed/24722424
http://dx.doi.org/10.1038/srep04659

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