High-efficiency RNA cloning enables accurate quantification of miRNA expression by deep sequencing

Small RNA cloning and sequencing is uniquely positioned as a genome-wide approach to quantify miRNAs with single-nucleotide resolution. However, significant biases introduced by RNA ligation in current protocols lead to inaccurate miRNA quantification by 1000-fold. Here we report an RNA cloning meth...

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Detalles Bibliográficos
Autores principales: Zhang, Zhaojie, Lee, Jerome E, Riemondy, Kent, Anderson, Emily M, Yi, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983620/
https://www.ncbi.nlm.nih.gov/pubmed/24098942
http://dx.doi.org/10.1186/gb-2013-14-10-r109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3983620/
https://www.ncbi.nlm.nih.gov/pubmed/24098942
http://dx.doi.org/10.1186/gb-2013-14-10-r109

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