Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry

We demonstrate an approach to optical DNA mapping, which enables near single-molecule characterization of whole bacteriophage genomes. Our approach uses a DNA methyltransferase enzyme to target labelling to specific sites and copper-catalysed azide-alkyne cycloaddition to couple a fluorophore to the...

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Detalles Bibliográficos
Autores principales: Vranken, Charlotte, Deen, Jochem, Dirix, Lieve, Stakenborg, Tim, Dehaen, Wim, Leen, Volker, Hofkens, Johan, Neely, Robert K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985630/
https://www.ncbi.nlm.nih.gov/pubmed/24452797
http://dx.doi.org/10.1093/nar/gkt1406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985630/
https://www.ncbi.nlm.nih.gov/pubmed/24452797
http://dx.doi.org/10.1093/nar/gkt1406

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