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Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry
We demonstrate an approach to optical DNA mapping, which enables near single-molecule characterization of whole bacteriophage genomes. Our approach uses a DNA methyltransferase enzyme to target labelling to specific sites and copper-catalysed azide-alkyne cycloaddition to couple a fluorophore to the...
Autores principales: | Vranken, Charlotte, Deen, Jochem, Dirix, Lieve, Stakenborg, Tim, Dehaen, Wim, Leen, Volker, Hofkens, Johan, Neely, Robert K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985630/ https://www.ncbi.nlm.nih.gov/pubmed/24452797 http://dx.doi.org/10.1093/nar/gkt1406 |
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