Correction of sequence-dependent ambiguous bases (Ns) from the 454 pyrosequencing system

Pyrosequencing of the 16S ribosomal RNA gene (16S) has become one of the most popular methods to assess microbial diversity. Pyrosequencing reads containing ambiguous bases (Ns) are generally discarded based on the assumptions of their non-sequence-dependent formation and high error rates. However,...

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Detalles Bibliográficos
Autores principales: Shin, Sunguk, Park, Joonhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985643/
https://www.ncbi.nlm.nih.gov/pubmed/24464999
http://dx.doi.org/10.1093/nar/gku070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985643/
https://www.ncbi.nlm.nih.gov/pubmed/24464999
http://dx.doi.org/10.1093/nar/gku070

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