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Correction of sequence-dependent ambiguous bases (Ns) from the 454 pyrosequencing system
Pyrosequencing of the 16S ribosomal RNA gene (16S) has become one of the most popular methods to assess microbial diversity. Pyrosequencing reads containing ambiguous bases (Ns) are generally discarded based on the assumptions of their non-sequence-dependent formation and high error rates. However,...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985643/ https://www.ncbi.nlm.nih.gov/pubmed/24464999 http://dx.doi.org/10.1093/nar/gku070 |