Identification of Ligand–Target Pairs from Combined Libraries of Small Molecules and Unpurified Protein Targets in Cell Lysates

[Image: see text] We describe the development and validation of interaction determination using unpurified proteins (IDUP), a method that selectively amplifies DNA sequences identifying ligand+target pairs from a mixture of DNA-linked small molecules and unpurified protein targets in cell lysates. B...

Descripción completa

Detalles Bibliográficos
Autores principales: McGregor, Lynn M., Jain, Tara, Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985698/
https://www.ncbi.nlm.nih.gov/pubmed/24495225
http://dx.doi.org/10.1021/ja412934t

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985698/
https://www.ncbi.nlm.nih.gov/pubmed/24495225
http://dx.doi.org/10.1021/ja412934t

Ejemplares similares