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Identification of Ligand–Target Pairs from Combined Libraries of Small Molecules and Unpurified Protein Targets in Cell Lysates

[Image: see text] We describe the development and validation of interaction determination using unpurified proteins (IDUP), a method that selectively amplifies DNA sequences identifying ligand+target pairs from a mixture of DNA-linked small molecules and unpurified protein targets in cell lysates. B...

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Detalles Bibliográficos
Autores principales:	McGregor, Lynn M., Jain, Tara, Liu, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3985698/ https://www.ncbi.nlm.nih.gov/pubmed/24495225 http://dx.doi.org/10.1021/ja412934t

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